Nā huahana
Eggs Drop Syndrome1976 virus Antibody ELISA Ki
Eggs Drop Syndrome1976 virus Antibody ELISA Ki
| Hōʻuluʻulu manaʻo | Ue ʻike i ka antibody kūʻē iā EDS76 i ka serum qualitatively. |
| Kumukānāwai | Hoʻohana ʻia ka pahu Eggs Drop Syndrome 1976 (EDS76) Ab Elisa kit no ka ʻike ʻana i ka antibody kūʻē iā EDS76 i ka serum qualitatively.No ka nānā ʻana i ka antibody ma hope o EDS76 immune a serological diagnostic of infection in Avian. |
| Nā Huhu ʻIke | Antibody kū'ē iā EDS76 i ka serum |
| Laʻana | Serum
|
| Ka nui | 1 pahu = 192 Hoao |
|
Paʻa a me ka waiho ʻana | 1) Pono e mālama ʻia nā reagents a pau ma 2 ~ 8 ℃.Mai maloʻo. 2) He 12 mahina ka noho ana.E hoʻohana i nā reagents a pau ma mua o ka lā pau o ka pahu.
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ʻIkepili
ʻO ka Egg Drop Syndrome (EDS-76) kahi maʻi maʻi maʻi i hoʻokumu ʻia e ka hui adenovirus III o ka genus Adenoviridae Avian Virus me ka hemagglutination.Ma kekahi mau mahiʻai moa, ua emi koke ka nui o ka hua manu o nā moa, a ua hana ʻia nā hua ʻino e like me nā hua manu ʻeleʻele, nā hua liʻiliʻi ʻole, a me nā hua manu ʻeleʻele i ka manawa like.ʻO ke kaʻina holoʻokoʻa o ka maʻi no 5-6 mau pule, ma hope o ka piʻi ʻana o ka hua hua manu, akā paʻakikī ke hiki i ka pae ma mua o ka emi ʻana.
Kumu o ka hoao ana
Ke hoʻohana nei kēia pahu i ke ʻano ELISA ʻole, hoʻomaʻemaʻe ʻia ka antigen EDS76 i uhi ʻia ma luna o nā ʻāpana micro-well enzyme.,inā loaʻa iā EDS76virusspecificantibody, e hui pū me ka antigen pre-coated, hoʻolei i ka antibody hui ʻole a me nā mea ʻē aʻe me ka holoi ʻana;a laila hoʻohui i ka enzyme labelled anti-EDS76 virus monoclonal antibody, a laila hui pū me ka antibody monoclonal a me ka antigen pre-coated;e hoʻolei i ka conjugate enzyme uncombined me ka holoi ʻana;E hoʻohui i ka substrate TMB i loko o nā pūnāwai micro, ʻo ka hōʻailona polū e Enzyme catalysis ma ke ʻano like ʻole o ka maʻiʻo antibody i loko o ka laʻana, e hoʻohana i ka mea heluhelu ELISA ma 450nm wavelenth e ana i ka waiwai o ka absorbance A ma nā pūnāwai hopena ma hope o ka hoʻohui ʻana i ka hopena hoʻomaha.
e hooki i ka pane.
ʻIkepili
| Reagent | Volume 96 Hoao/192 Hoao | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | serum dilution microplate | 1ea/2ea | |
| 11 | Aʻo | 1 pcs |
