ʻO ka maʻi ʻo Newcastle Antibody ELISA Kit

Ke hoʻohana nei kēia pahu i ke ala ELISA poloka, ua uhi mua ʻia ka antigen NDV ma ka microplate.I ka hoʻāʻo ʻana, e hoʻohui i kahi laʻana serum diluted, ma hope o ka incubation, inā he NDV kikoʻī antibody, e hui pū me ka antigen pre-coated, e hoʻolei i ka antibody uncombined a me nā mea ʻē aʻe me ka holoi ʻana;a laila hoʻohui i ka enzyme i hoʻopaʻa ʻia i ka anti-NDV monoclonal antibody, antibody i loko o ka laʻana poloka ka hui pū ʻana o ka antibody monoclonal a me ka antigen pre-coated;e hoʻolei i ka hui ʻana o ka enzyme uncombined me ka holoi ʻana. Hoʻohui i ka substrate TMB i loko o nā pūnāwai liʻiliʻi, ʻo ka hōʻailona polū e Enzyme catalysis ma ke ʻano like ʻole o ka ʻike antibody i ka laʻana.