ʻO ka moa Infectious Bursal Disease Virus Ab Elisa kit

Code Huahana:

Mea inoa: Chicken Infectious Bursal Disease Virus Ab Elisa kit

Hōʻuluʻulu manaʻo: Chicken infectious bursal disease virus antibody detection kit no ka ʻike ʻana i ka moa infectious bursal maʻi virus neutralizing antibody i loko o ka moa serum no ka loiloi ʻana i nā antibodies neutralizing i hana ʻia e ka moa infectious bursal maʻi kano kano kano kūlana a me ka serologically assisted diagnosis o nā moa maʻi.

Nā Manaʻo ʻImi: ʻAi ʻiʻo maʻi maʻi maʻi maʻi maʻi moa

Laana Ho'āʻo: Serum

Hōʻike: 1 pahu = 192 Hōʻike

Ka mālama ʻana: Pono e mālama ʻia nā reagents a pau ma 2 ~ 8 ℃.Mai maloʻo.

Manawa Kau: 12 mahina.E hoʻohana i nā reagents a pau ma mua o ka lā pau o ka pahu.

E hoʻouna i ka leka uila iā mākou KĀLĀKĀ

Huahana Huahana

ʻO ka moa Infectious Bursal Disease Virus Ab Elisa kit

Hōʻuluʻulu manaʻo	ʻO ka ʻike ʻana o ka neutralizing antibody e kūʻē i ka bursa infectious o Fabricius virus i ka serum moa
Nā Huhu ʻIke	ʻO ka moa infectious bursal disease virus antibody
Laʻana	Serum
Ka nui	1 pahu = 192 Hoao
Paʻa a me ka waiho ʻana	1) Pono e mālama ʻia nā reagents a pau ma 2 ~ 8 ℃.Mai maloʻo. 2) He 12 mahina ka noho ana.E hoʻohana i nā reagents a pau ma mua o ka lā pau o ka pahu.

ʻIkepili

ʻO ka maʻi maʻi lele(IBD), i kapa ʻia ʻo Gumboro maʻi, infectious bursitis a me infectious avian nephrosis, he maʻi lele nui o nā ʻōpio.moaa me nā turkey i hoʻokumu ʻia e ka maʻi maʻi bursal infectious (IBDV), i hōʻike ʻia eimmunosuppressiona me ka make maʻamau ma 3 a 6 pule o ka makahiki.Ua ʻike mua ʻia ka maʻi maGumboro, Delawarei 1962. He mea koʻikoʻi ia i ka ʻoihana moa ma ka honua holoʻokoʻa ma muli o ka piʻi ʻana o ka maʻi i nā maʻi ʻē aʻe a me ka hoʻopilikia maikaʻi ʻole ʻana me ka maikaʻi.lāʻau lapaʻau.I nā makahiki i hala iho nei, ua puka mai nā maʻi ʻino loa o IBDV (vvIBDV), ka mea e make nui ai ka moa, ma ʻEulopa,ʻAmelika Latina,Hikina Hema ʻAsia, ʻApelika a me kaHikina Waena.Loaʻa ka maʻi ma ke ala oro-fecal, me ka manu i hoʻopilikia ʻia e hoʻokuʻu i nā pae kiʻekiʻe o ka maʻi ma kahi o 2 pule ma hope o ka maʻi.Hoʻolaha maʻalahi ka maʻi mai nā moa maʻi i nā moa olakino ma o ka meaʻai, ka wai, a me ka pili kino.

Kumu o ka hoao ana

Ke hoʻohana nei ka pahu i kahi ʻano hoʻokūkū ELISA, i hoʻopaʻa mua ʻia i ka maʻi bursal maʻi virus VP2 protein ma ka microplate, a hoʻokūkū me ka anti-VP2 protein antibody i loko o ka serum no ka vector phase paʻa e hoʻohana ana i ka anti-VP2 protein monoclonal antibody.I ka ho'āʻo, he monoclonal antibody e ho'āʻo a me ka anti-VP2 pūmua, a ma hope o incubation, ina ka hāpana i loko o ka moa infectious bursal maʻi virus VP2 protein-specific antibody, ua nakinaki ia i ka antigen ma luna o ka pā uhi.Ma laila e pale ai i ka paʻa ʻana o ka anti-VP2 protein monoclonal antibody i ka antigen, ma hope o ka holoi ʻana e wehe i ka antibody unbound a me nā mea ʻē aʻe;a laila hoʻohui i kahi anti-mouse enzyme-labeled secondary antibody e hoʻopaʻa pono i ka paʻakikī antigen-antibody ma ka pā ʻike;Hoʻopau ʻia ka conjugate enzyme unbound ma ka holoi ʻana;ua hoʻohui ʻia ka substrate TMB i ka microwell e hoʻomohala i ke kala, a ua hoʻopili maikaʻi ʻia ka waiwai absorbance o ka hāpana me ka ʻike o ka anti-VP2 protein antibody i loko, a laila e hoʻokō ai i ke kumu o ka ʻike ʻana i ka anti-VP2 protein antibody i ka hāpana.