ʻAno maʻi wāwae a me ka waha ʻO Ab ELISA pahu hoʻāʻo kiʻi i hōʻike ʻia

ʻAno hoʻāʻo ʻo Ab ELISA i ka maʻi wāwae a me ka waha

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ʻAno hoʻāʻo ʻo Ab ELISA i ka maʻi wāwae a me ka waha

Hōʻuluʻulu manaʻo	ʻIke ʻia o nā kikoʻīWae a me ka waha antibodyʻAno O
Kumukānāwai	Hoʻohana ka FMD Type Asia I antibody ELISA test kit no ka ʻike ʻana i nā antibodies maʻi maʻi wāwae-a-waha i loko o ka serum o nā puaʻa, pipi, hipa a me nā kao no ka loiloi ʻana i ka pale ʻana i ka maʻi FMD.
Nā Huhu ʻIke	Wae a me ka waha antibodyʻAno O
Laʻana	Serum
Ka nui	1 pahu = 192 Hoao
Paʻa a me ka waiho ʻana	1) Pono e mālama ʻia nā reagents a pau ma 2 ~ 8 ℃.Mai maloʻo. 2) He 12 mahina ka noho ana.E hoʻohana i nā reagents a pau ma mua o ka lā pau o ka pahu.

ʻIkepili

ʻO ka maʻi maʻi wāwae a me ka waha(FMDV) ʻo ia kapathogenke kumuka maʻi wāwae a me ka waha.^[1]He apicornavirus, ka lālā prototypical o ka genusAphthovirus.ʻO ka maʻi, ke kumu o nā vesicles (blisters) ma ka waha a me nā wāwae opipi, puaa, hipa, kao, a me na mea e aemāiʻuʻuHe ma'i lele nui na holoholona a he ahulau nui omahiʻai holoholona

Kumu o ka hoao ana

Hoʻohana ʻia kēia ʻano hana ELISA, hoʻomaʻemaʻe FMDVantigenis i uhi ʻia ma nā ʻāpana micro-well enzyme.I ka hoʻāʻo ʻana, hoʻohui ʻia ka laʻana serum, ma hope o ka incubation, inā loaʻa ka maʻi virus FMD kūikawā, e hui pū me ka antigen pre-coated, e hoʻolei i ka antibody uncombined a me nā mea ʻē aʻe me ka holoi ʻana;a laila e hoʻohui i ka enzyme conjugate, e hoʻolei i ka conjugate enzyme uncombined me ka holoi.Hoʻohui i ka substrate TMB i loko o nā pūnāwai liʻiliʻi, ʻo ka hōʻailona polū e Enzyme catalysis ka hoʻohālikelike pololei o ka ʻike antibody i ka laʻana.