Hōʻuluʻulu manaʻo | ʻO ka ʻike ʻana i nā ʻano antibody Bovine Tuberculosis (BTB). |
Kumukānāwai | Hiki ke hoʻohana ʻia ka pahu hōʻike hōʻike hōʻike ʻo Bovine Tuberculosis (BTB) e ʻike ai i ka antibody Bovine Tuberculosis i ka serum a i ʻole plasma o Bovine. |
Nā Huhu ʻIke | ʻO ka maʻi maʻi Bovine (BTB) antibody |
Laʻana | Serum
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Ka nui | 1 pahu = 192 Hoao |
Paʻa a me ka waiho ʻana | 1) Pono e mālama ʻia nā reagents a pau ma 2 ~ 8 ℃.Mai maloʻo. 2) He 12 mahina ka noho ana.E hoʻohana i nā reagents a pau ma mua o ka lā pau o ka pahu.
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ʻO ka Mycobacterium bovis he mea ulu lohi (16- a hiki i ka 20-hola hanauna manawa) aerobic a me ke kumu kumu o ka maʻi maʻi i loko o nā pipi (i ʻike ʻia he TB bovine).Pili ia i ka Mycobacterium tuberculosis, ka maʻi bacteria e hoʻoulu ai i ka maʻi maʻi i loko o ke kanaka.Hiki iā M. bovis ke lele i ka pale ʻano a hoʻoulu i ka maʻi e like me ka maʻi maʻi i loko o ke kanaka a me nā mea momona ʻē aʻe.
ʻO kēia kit hoʻohana kū ʻole ELISA ʻano hana, hoomaemae BTB antigen is i uhi mua ʻia on enzyme micro-well nā kāʻei. Ke hoʻāʻo, hoʻohui diluted serum laʻana, mahope incubation, if ma laila is BTB kikoʻī antibody, it makemake hui pu me ka i uhi mua ʻia antigen, hoolei ka hui ole antibody a ʻē aʻe ʻāpana me holoi; alaila hoʻohui enzyme conjugate, hoolei ka hui ole enzyme conjugate me
holoi. Hoʻohui i ka substrate TMB i loko o nā pūnāwai micro, pololei ka hōʻailona polū e Enzyme catalysis ka māhele o ka mea antibody ma ka laʻana.
Reagent | Volume 96 Hoao/192 Hoao | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Aʻo | 1 pcs |