Hōʻuluʻulu manaʻo | Ka ʻike ʻana i ka Antibody kūikawā o nā Peste Des Petits Ruminants |
Kumukānāwai | Hoʻohana ʻia ka pahu hoʻāʻo ELISA antibody PPRV no ka ʻike ʻana i nā antibodies maʻi Peste des petits ruminants i loko o ka serum o nā hipa a me nā kao. |
Nā Huhu ʻIke | PPRV antibody |
Laʻana | Serum
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Ka nui | 1 pahu = 192 Hoao |
Paʻa a me ka waiho ʻana | 1) Pono e mālama ʻia nā reagents a pau ma 2 ~ 8 ℃.Mai maloʻo. 2) He 12 mahina ka noho ana.E hoʻohana i nā reagents a pau ma mua o ka lā pau o ka pahu.
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Ovine rinderpest, i ʻike nui ʻia ʻo iapeste des petits ruminants(PPR), he ma'i lele e pili ananā kaoahipa;akā, ʻo nā kāmelo a me nā liʻiliʻi hihiunā mea hānai holoholonahiki ke pili.Aia ka PPR i kēia manawaKe akau, Waena, KomohanaaʻApelika Hikina, kaHikina Waena, aAsia Hema. Hoʻokumu ʻia eliʻiliʻi ruminants morbillivirusi loko o ka genusʻO Morbillivirus,a pili loa me kekahi, ʻo rinderpest morbillivirus, maʻi ʻaʻai morbillivirus, aʻīlio mobillivirus(i ʻike mua ʻia ʻoʻīliovirus distemper).He maʻi lele loa ka maʻi, a hiki ke loaʻa i ka 80-100% ka nui o ka make maʻoʻoleʻahihia ma kahiepizootichoʻonohonoho.ʻAʻole pili ka maʻi i ke kanaka.
ʻIke ʻia ʻo PPR ma ke ʻano he maʻi kao,kata, ma'i o ka stomatitis-pneumoenteritis, a me ka ovine rinderpest.
ʻO nā keʻena kūhelu e like me kaFAOaOIEhoʻohana i ka inoa Farani "peste des petits ruminants" me nā ʻano ʻokoʻa spelling.
Ke hoʻohana nei kēia pahu i ke ʻano hoʻokūkū ELISA e uhi mua i nā antigens PPRV ma nā pūnāwai microplate.I ka hoʻāʻo ʻana, hoʻohui i kahi laʻana serum diluted, ma hope o ka incubation, inā loaʻa iā PPRV antibody, e hui pū me ka antigen pre-coated, antibody i ka laʻana poloka i ka hui pū ʻana o ka antibody monoclonal a me ka antigen pre-coated;e hoʻolei i ka conjugate enzyme uncombined me ka holoi ʻana;Hoʻohui i ka substrate TMB i loko o nā pūnāwai liʻiliʻi, ʻo ka hōʻailona polū e Enzyme catalysis ma ke ʻano like ʻole o ka ʻike antibody i ka laʻana.
Reagent | Volume 96 Hoao/192 Hoao | ||
1 |
| 1ea/2ea | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Aʻo | 1 pcs |