Hōʻuluʻulu manaʻo | ʻIke ʻia o nā kikoʻīWae a me ka waha antibodyʻAno O |
Kumukānāwai | Hoʻohana ka FMD Type Asia I antibody ELISA test kit no ka ʻike ʻana i nā antibodies maʻi maʻi wāwae-a-waha i loko o ka serum o nā puaʻa, pipi, hipa a me nā kao no ka loiloi ʻana i ka pale ʻana i ka maʻi FMD. |
Nā Huhu ʻIke | Wae a me ka waha antibodyʻAno O |
Laʻana | Serum
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Ka nui | 1 pahu = 192 Hoao |
Paʻa a me ka waiho ʻana | 1) Pono e mālama ʻia nā reagents a pau ma 2 ~ 8 ℃.Mai maloʻo. 2) He 12 mahina ka noho ana.E hoʻohana i nā reagents a pau ma mua o ka lā pau o ka pahu.
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ʻO ka maʻi maʻi wāwae a me ka waha(FMDV) ʻo ia kapathogenke kumuka maʻi wāwae a me ka waha.[1]He apicornavirus, ka lālā prototypical o ka genusAphthovirus.ʻO ka maʻi, ke kumu o nā vesicles (blisters) ma ka waha a me nā wāwae opipi, puaa, hipa, kao, a me na mea e aemāiʻuʻuHe ma'i lele nui na holoholona a he ahulau nui omahiʻai holoholona
Hoʻohana ʻia kēia ʻano hana ELISA, hoʻomaʻemaʻe FMDVantigenis i uhi ʻia ma nā ʻāpana micro-well enzyme.I ka hoʻāʻo ʻana, hoʻohui ʻia i ka laʻana serum, ma hope o ka incubation, inā loaʻa ka FMD virus kikoʻī antibody, e hui pū me ka antigen pre-coated, e hoʻolei i ka antibody uncombined a me nā mea ʻē aʻe me ka holoi ʻana;a laila e hoʻohui i ka enzyme conjugate, e hoʻolei i ka hui ʻole o ka enzyme me ka holoi ʻana.Hoʻohui i ka substrate TMB i loko o nā pūnāwai liʻiliʻi, ʻo ka hōʻailona polū e Enzyme catalysis ka hoʻohālikelike pololei o ka ʻike antibody i ka laʻana.
Reagent | Volume 96 Hoao/192 Hoao | ||
1 |
| 1ea/2ea | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Aʻo | 1 pcs |