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ʻAno hoʻāʻo ʻo Ab ELISA i ka maʻi wāwae a me ka waha

Code Huahana:

Inoa Mea: ʻAno maʻi wāwae a me ka waha ʻO Antibody ELISA Test Kit

Hōʻuluʻulu manaʻo: Hoʻohana ka FMD Type O antibody ELISA pahu hoʻāʻo no ka ʻike ʻana i nā antibodies maʻi maʻi wāwae-a-waha i loko o ka serum o nā puaʻa, pipi, hipa a me ke kao no ka loiloi ʻana i ka pale ʻana i ka maʻi FMD.

Nā Manaʻo ʻIke: ʻO ka maʻi wāwae a me ka waha ke ʻano o Antibody

Laana Ho'āʻo: Serum

Hōʻike: 1 pahu = 192 Hōʻike

Ka mālama ʻana: Pono e mālama ʻia nā reagents a pau ma 2 ~ 8 ℃.Mai maloʻo.

Ka manawa mālama: 12 mahina.E hoʻohana i nā reagents a pau ma mua o ka lā pau o ka pahu.


Huahana Huahana

Huahana Huahana

ʻAno hoʻāʻo ʻo Ab ELISA i ka maʻi wāwae a me ka waha

Hōʻuluʻulu manaʻo ʻIke ʻia o nā kikoʻīWae a me ka waha antibodyʻAno O
Kumukānāwai

Hoʻohana ka FMD Type Asia I antibody ELISA test kit no ka ʻike ʻana i nā antibodies maʻi maʻi wāwae-a-waha i loko o ka serum o nā puaʻa, pipi, hipa a me nā kao no ka loiloi ʻana i ka pale ʻana i ka maʻi FMD.

Nā Huhu ʻIke Wae a me ka waha antibodyʻAno O
Laʻana Serum

 

Ka nui 1 pahu = 192 Hoao
 

 

Paʻa a me ka waiho ʻana

1) Pono e mālama ʻia nā reagents a pau ma 2 ~ 8 ℃.Mai maloʻo.

2) He 12 mahina ka noho ana.E hoʻohana i nā reagents a pau ma mua o ka lā pau o ka pahu.

 

 

 

ʻIkepili

ʻO ka maʻi maʻi wāwae a me ka waha(FMDV) ʻo ia kapathogenke kumuka maʻi wāwae a me ka waha.[1]He apicornavirus, ka lālā prototypical o ka genusAphthovirus.ʻO ka maʻi, ke kumu o nā vesicles (blisters) ma ka waha a me nā wāwae opipi, puaa, hipa, kao, a me na mea e aemāiʻuʻuHe ma'i lele nui na holoholona a he ahulau nui omahiʻai holoholona

Kumu o ka hoao ana

Hoʻohana ʻia kēia ʻano hana ELISA, hoʻomaʻemaʻe FMDVantigenis i uhi ʻia ma nā ʻāpana micro-well enzyme.I ka hoʻāʻo ʻana, hoʻohui ʻia i ka laʻana serum, ma hope o ka incubation, inā loaʻa ka FMD virus kikoʻī antibody, e hui pū me ka antigen pre-coated, e hoʻolei i ka antibody uncombined a me nā mea ʻē aʻe me ka holoi ʻana;a laila e hoʻohui i ka enzyme conjugate, e hoʻolei i ka hui ʻole o ka enzyme me ka holoi ʻana.Hoʻohui i ka substrate TMB i loko o nā pūnāwai liʻiliʻi, ʻo ka hōʻailona polū e Enzyme catalysis ka hoʻohālikelike pololei o ka ʻike antibody i ka laʻana.

ʻIkepili

 

Reagent

Volume

96 Hoao/192 Hoao

1
ʻO ka microplate i uhi ʻia i ka antigen

 

1ea/2ea

2
Manaʻo ʻino

 

2ml

3
Manaʻo maikaʻi

 

1.6ml

4
Nā mea hoʻoheheʻe laʻana

 

100ml

5
ʻO ka mea holoi holoi (10X hoʻopaʻa ʻia)

 

100ml

6
Hoʻohui ʻia ka enzyme

 

11/22ml

7
Pāpaʻa

 

11/22ml

8
Haʻina hoʻomaha

 

15ml

9
Mea hoʻopaʻa paʻa paʻa

 

2ea/4ea

10 serum dilution microplate

1ea/2ea

11 Aʻo

1 pcs

 


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