ʻO ka maʻi wāwae a me ka waha ʻAno A Antibody ELISA pahu hoʻāʻo

Ke hoʻohana nei kēia pahu i ke ʻano hoʻokūkū ELISA no ka uhi mua ʻana i nā antigens maʻi maʻi wāwae-a-waha ma nā luawai microplate.I ka hoʻāʻo ʻana, hoʻohui i ka laʻana serum diluted a me ka anti-FMD Ab, ma hope o ka incubation, inā loaʻa iā FMD antibody, e hui pū me ka antigen pre-coated, antibody i ka laʻana poloka ka hui pū ʻana o ka anti-FMD antibody a me ka antigen pre-coated;e hoʻolei i ka conjugate enzyme uncombined me ka holoi ʻana;Hoʻohui i ka substrate TMB i loko o nā pūnāwai liʻiliʻi, ʻo ka hōʻailona polū e Enzyme catalysis ma ke ʻano like ʻole o ka ʻike antibody i ka laʻana.