Hōʻuluʻulu manaʻo | Hoʻohana ʻia e ʻike i nā antibodies kūikawā e kūʻē i ka maʻi avian influenza (AIV) i loko o ka serum |
Kumukānāwai | Hoʻohana ʻia ʻo Avian influenza antibody Elisa kit no ka ʻike ʻana i ka antibody kūʻē i ka Avian influenza Virus (AIV) i loko o ka serum, no ka nānā ʻana i ka antibody ma hope o ka pale ʻana o AIV a me ka serological diagnostic of infection in Avian. |
Nā Huhu ʻIke | ʻAi ʻAi ʻAi ʻAi Maʻi ʻAi |
Laʻana | Serum
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Ka nui | 1 pahu = 192 Hoao |
Paʻa a me ka waiho ʻana | 1) Pono e mālama ʻia nā reagents a pau ma 2 ~ 8 ℃.Mai maloʻo. 2) He 12 mahina ka noho ana.E hoʻohana i nā reagents a pau ma mua o ka lā pau o ka pahu.
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ʻO ka maʻi maʻi Avian, i ʻike ʻole ʻia ʻo ka maʻi avian a i ʻole ka maʻi manu, he ʻano maʻi maʻi i hoʻokumu ʻia e nā maʻi i hoʻololi ʻia imanu.
ʻO ke ʻano me ka pilikia nui loa, ʻo ia ka maʻi avian maʻi pathogenic (HPAI).Ua like ka ma'i manu memaʻi maʻi puaʻa, ma'i 'īlio, lio
ʻO ka maʻi maʻi a me ka maʻi maʻi kanaka ma ke ʻano he maʻi i hoʻokumu ʻia e nā maʻi maʻi influenza i hoʻololi ʻia i kahi kikoʻī
hoʻokipa.Mai loko mai o nā ʻano maʻi maʻi influenza ʻekolu (A,B, aC), ʻo ka maʻi influenza A hezoonoticka maʻi me kahi mea kūlohelohe
ʻaneʻane piha i loko o nā manu.ʻO ka maʻi maʻi Avian, no ka nui o nā kumu, pili i ka maʻi influenza A.
Ke hoʻohana nei kēia pahu i ke ala ELISA poloka, ua uhi mua ʻia ka antigen AIV ma ka microplate.I ka hoʻāʻo ʻana, e hoʻohui i kahi laʻana serum diluted, ma hope o ka incubation, inā he AIV kūikawā antibody, e hui pū me ka antigen pre-coated, e hoʻolei i ka antibody uncombined a me nā mea ʻē aʻe me ka holoi;a laila hoʻohui i ka enzyme i hoʻopaʻa ʻia i ka anti-AIV monoclonal antibody, antibody i loko o ka laʻana poloka i ka hui pū ʻana o ka antibody monoclonal a me ka antigen pre-coated;e hoʻolei i ka hui ʻana o ka enzyme uncombined me ka holoi ʻana.Hoʻohui i ka substrate TMB i loko o nā pūnāwai liʻiliʻi, ʻo ka hōʻailona polū e Enzyme catalysis ma ke ʻano like ʻole o ka ʻike antibody i ka laʻana.
Reagent | Volume 96 Hoao/192 Hoao | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Aʻo | 1 pcs |