Anaplasma Phagocytophilum Ab pahu ho'āʻo | |
Helu helu | RC-CF26 |
Hōʻuluʻulu manaʻo | Ka ʻike ʻana i nā antibodies kikoʻī o Anaplasmai loko o 10 mau minuke |
Kumukānāwai | Hoʻokahi ʻanuʻu immunochromatographic assay |
Nā Huhu ʻIke | Anaplasma antibodies |
Laʻana | ʻO ke koko holoʻokoʻa, serum a plasma paha |
Heluhelu manawa | 5~ 10 minuke |
ʻO ka ʻike | 100.0 % vs. IFA |
kikoo | 100.0 % vs. IFA |
Ka palena o ka ike | IFA Titer 1/16 |
Ka nui | 1 pahu (kit) = 10 mau mea hana (Paʻi kanaka) |
ʻIkepili | ʻO ka pahu hoʻāʻo, ka ʻōmole Buffer, a me nā mea hoʻolele hoʻolele |
E akahele | E hoʻohana i loko o 10 mau minuke ma hope o ka wehe ʻanaE hoʻohana i ka nui kūpono o ka laʻana (0.01 ml o kahi dropper) E hoʻohana ma hope o 15 ~ 30 mau minuke ma RT inā mālama ʻia lākou ma lalo o ke anuanu E noʻonoʻo i ka hopena o ka hoʻāʻo ʻana ma hope o 10 mau minuke |
Hiki i ka ma'i ma'i Anaplasma phagocytophilum (Ehrilichia phagocytophila ma mua) ke ho'oulu i ka ma'i i kekahi mau holoholona me ke kanaka.ʻO ka maʻi i loko o nā ruminants home ua kapa ʻia hoʻi ʻo tick-borne fever (TBF), a ua ʻike ʻia no ka liʻiliʻi he 200 mau makahiki.ʻO nā maʻi bacteria o ka ʻohana Anaplasmataceae he gram-negative, nonmotile, coccoid a ellipsoid organisms, ʻokoʻa ka nui mai 0.2 a 2.0um anawaena.He aerobes obligate lākou, nele i ke ala glycolytic, a he mau parasites intracellular obligate.Noho nā ʻano ʻano āpau i loko o ka genus Anaplasma i nā vacuoles i hoʻopaʻa ʻia me ka membrane i loko o nā pūnaeho hematopoietic ʻoi ʻole a oʻo paha o ka host mammalian.Hoʻopili ka phagocytophilum i nā neutrophils a ʻo ka huaʻōlelo granulocytotropic e pili ana i nā neutrophils maʻi.ʻAʻole i loaʻa nā mea ola i loko o nā eosinophils.
Anaplasma phagocytophilum
ʻO nā hōʻailona lapaʻau maʻamau o ka anaplasmosis canine e pili ana i ke kuni kiʻekiʻe, ka lethargy, ke kaumaha a me ka polyarthritis.Hiki ke ʻike ʻia nā hōʻailona neurologic (ataxia, hopu a me ka ʻeha ʻāʻī).ʻO ka maʻi Anaplasma phagocytophilum ʻaʻole hiki ke make ke ʻole ka paʻakikī e nā maʻi ʻē aʻe.Ua ʻike ʻia nā poho pololei, nā kūlana ʻōʻopa a me nā poho hana i nā keiki hipa.Ua hoʻopaʻa ʻia ka hoʻopaʻa ʻana a me ka spermatogenesis maikaʻi ʻole i nā hipa a me nā pipi.Hoʻopili ʻia ka paʻakikī o ka maʻi e kekahi mau kumu, e like me nā ʻano like ʻole o Anaplasma phagocytophilum i pili, nā pathogens ʻē aʻe, ka makahiki, ke kūlana immune a me ke kūlana o ka mea hoʻokipa, a me nā mea e like me ke aniau a me ka hoʻokele.Pono e ʻōlelo ʻia ʻo nā hōʻike lapaʻau i loko o ke kanaka mai kahi maʻi maʻi like ʻole e like me ka maʻi, a i kahi maʻi hoʻoweliweli.Eia nō naʻe, ʻo ka hapa nui o nā maʻi maʻi kanaka e hopena i ka liʻiliʻi a i ʻole nā hōʻike lapaʻau.
Hoʻouna ʻia ʻo Anaplasma phagocytophilum e nā ticks ixodid.Ma ʻAmelika Hui Pū ʻIa, ʻo Ixodes scapularis a me Ixodes pacificus nā vectors nui, ʻoiai ʻo Ixode ricinus i ʻike ʻia ʻo ia ka vector exophilic nui ma ʻEulopa.Hoʻouna ʻia ʻo Anaplasma phagocytophilum e kēia mau ʻano vector ticks, a ʻaʻohe hōʻike o ka transovarial transmission.ʻO ka hapa nui o nā haʻawina a hiki i kēia lā e noiʻi ana i ke koʻikoʻi o nā pūʻali mammalian o A. phagocytophilum a me kāna mau mea hoʻokalakupua i kālele i nā rodents akā aia kēia mea ola i ka laulā o nā mea hoʻokipa mammalian, e hoʻopilikia ana i nā pōpoki hānai, nā ʻīlio, nā hipa, nā bipi, a me nā lio.
ʻO ka indirect immunofluorescence assay ka hoʻāʻo nui i hoʻohana ʻia e ʻike i ka maʻi.Hiki ke loiloi ʻia nā laʻana serum acute a convalescent no ka huli ʻana i kahi hoʻololi ʻehā o ka titer antibody i Anaplasma phagocytophilum.ʻIke ʻia nā mea hoʻokomo intracellular (morulea) i loko o nā granulocytes ma Wright a i ʻole Gimsa stained blood smears.Hoʻohana ʻia nā ʻano hana polymerase chain reaction (PCR) e ʻike i ka DNA Anaplasma phagocytophilum.
ʻAʻohe lāʻau lapaʻau no ka pale ʻana i ka maʻi Anaplasma phagocytophilum.ʻO ka pale ʻana i ka pale ʻana i ka ʻike ʻana i ka vector tick (Ixodes scapularis, Ixodes pacificus, a me Ixode ricinus) mai ka pūnāwai a hiki i ka hāʻule, hoʻohana prophylatic o nā antiacaricides, a me ka hoʻohana prophylactic o doxycycline a i ʻole tetracycline ke kipa aku iā Ixodes scapularis, Ixodes pacificus, a me Ixode ricinus. nā ʻāina endemic.