Nā huahana
Lifecosm Canine Brucellosis Ag Mea Ho'āʻo wikiwiki
LSH Ab Hoao
| ʻO ka pahu ho'āʻo Brucella Ag | |
| Helu helu | RC-CF10 |
| Hōʻuluʻulu manaʻo | Ka ʻike ʻana i ka antigen kūikawā o Brucella i loko o 10 mau minuke |
| Kumukānāwai | Hoʻokahi ʻanuʻu immunochromatographic assay |
| Nā Huhu ʻIke | Brucella antigen |
| Laʻana | Canine, bovine a me Ovis Whole Blood, Plasma a Serum paha |
| Heluhelu manawa | 10 ~ 15 minuke |
| ʻO ka ʻike | 91.3 % vs. IFA |
| kikoo | 100.0 % vs. IFA |
| Ka palena o ka ike | IFA Titer 1/16 |
| Ka nui | 1 pahu (kit) = 10 mau mea hana (Paʻi kanaka) |
| ʻIkepili | pahu ho'āʻo, nā paipu, nā mea hoʻolele hoʻolele |
|
E akahele | E hoʻohana i loko o 10 mau minuke ma hope o ka wehe ʻana E hoʻohana i ka nui kūpono o ka laʻana (0.01 ml o kahi dropper) E hoʻohana ma hope o 15 ~ 30 mau minuke ma RT inā mālama ʻia lākou ma lalo o ke anuanu E noʻonoʻo i ka hopena o ka hoʻāʻo ʻana ma hope o 10 mau minuke |
ʻIkepili
He lālā ka genus Brucella o ka ʻohana Brucellaceae a he ʻumi mau ʻano ʻano liʻiliʻi, non-motile, non-sporing, aerobic, gram-negative intracellular coccobacilli.ʻO lākou ka catalase, oxidase a me ka urea maikaʻi bacteria.Hiki i nā lālā o ka genus ke ulu ma nā media i hoʻonui ʻia e like me blood agar a i ʻole chocolate agar.ʻO ka Brucellosis kahi zoonosis kaulana, aia ma nā ʻāina āpau, akā me ka nui o ka maʻi maʻi a me nā hanana, i nā holoholona a me nā kānaka.ʻO Brucella, ma ke ʻano o nā parasite intracellular facultative, hoʻopaʻa i nā ʻano holoholona lehulehu i kahi ala mau loa, a mau loa paha, no ko lākou ola holoʻokoʻa.
ʻO Brucella colony helehelena
Hoʻouna ʻia
Hoʻouna pinepine ʻia nā ʻano Brucella ma waena o nā holoholona ma o ka hoʻopili ʻana me ka placenta, fetus, fetal fluids a me ka hoʻokuʻu ʻana o ka wahine.he holoholona ma'i.Loaʻa ka hapa nui a i ʻole nā ʻano Brucella a pau i loko o ka pua.Hiki i ke kāne ke hoʻokahe i kēia mau mea ola no ka wā lōʻihi a i ʻole ke ola.Ua ʻike ʻia kekahi mau ʻano Brucella i loko o nā mea huna a me nā excretions ʻē aʻe e like me ka mimi, feces, ka wai hygroma, salvia, ka waiū a me nā huna huna a me ka ocular.
Ecology o nā maʻi zoonotic Brucella
Nā hōʻailona
I nā bipi
ʻAʻohe ala kūpono e ʻike ai i nā holoholona maʻi ma ko lākou helehelena.ʻO nā hōʻailona maopopo loa o nā holoholona hāpai, ʻo ia ka hoʻohemo ʻana a i ʻole ka hānau ʻana o nā keiki bipi nāwaliwali.Hiki ke hoʻemi ʻia ka hana ʻana o ka waiū ma muli o ka hoʻololi ʻana i ka wā lactation maʻamau i hoʻokumu ʻia e ka hāpai ʻana a me ka hoʻopaneʻe ʻana.ʻO nā hōʻailona ʻē aʻe o ka brucellosis, ʻo ia ka emi ʻana o ka momona me ka liʻiliʻi o ka hānau ʻana, mālama ʻia ka hānau ʻana me ka hopena o nā maʻi ʻōpū, a (i kekahi manawa) hoʻonui ʻia, nā hui ʻāʻī.
I nā ʻīlio
I nā ʻīlio, noho maʻamau ka maʻi bacteria Brucellosis i loko o nā genital a me ka ʻōnaehana lymphatic, akā hiki ke laha i nā puʻupaʻa, nā maka a me ka disc intervertebral pū kekahi.Ke hoʻopili ʻo Brucellosis i ka disc intervertebral, ʻo ka hopena he discospondylitis.I loko o nā ʻīlio, maʻa mau nā hōʻailona mai nā ʻōpū hānau.Hiki i nā ʻīlio kāne ke hoʻomohala i ka maʻi scrotal a me ka testicular, ʻoiai hiki i nā ʻīlio wahine ke hāʻule ʻole.He mea maʻamau ke kuni, akā ʻo ka ʻeha e pili ana me Brucellosis hiki ke hoʻonāwaliwali i ka ʻīlio.Inā laha ka maʻi i nā puʻupaʻa, hiki i nā maka a i ʻole nā hōʻailona intervertebral disc ke hoʻomaka e hōʻike mai kēia mau kino.
I ka puaʻa
ʻO ka manawa ma waena o ka maʻi a me ka ʻike ʻia ʻana o nā hōʻailona o ka maʻi mai ka 1 pule a i ka 2 mahina.ʻO nā hōʻailona i loaʻa i ka maʻi puaʻa, ʻo ia ka hapa o ka hānau ʻana - ka hoʻihoʻi ʻana i ka lawelawe ma hope o ka male ʻana a me ka hānau ʻana o nā puaʻa nāwaliwali a hānau ʻole paha.Hiki i kekahi mau lūlū ke ulu i kahi maʻi o ka ʻōpū a hōʻike i kahi hoʻokuʻu kino.Hiki i nā puaʻa maʻi ke hoʻoulu i ka pehu a me ka pehu.Hiki i nā kāne ʻelua ke lilo i ʻoʻopa me ka pehu ʻana o nā hono a/a i ʻole e hoʻomohala i nā hōʻailona o ka hui ʻole a me ka lolo o ka wāwae hope.
ʻIkepili
1. Ka hoʻokaʻawale ʻana a me ka ʻike ʻana o ka mea hana
Hiki ke hoʻihoʻi ʻia nā ʻano ʻano Brucella mai nā ʻiʻo he nui a me nā mea huna, ʻo ia hoʻi nā membrane fetal, nā mea huna o ka wahine, ka waiū (a i ʻole nā mea huna o ka udder), semen, arthritis o ka wai hygroma, a me ka ʻōpū o ka ʻōpū, ka spleen a me ka māmā mai nā keiki hānai.ʻO ka hapa nui o nā ʻano Brucella mai nā kolone i loko o kekahi mau lā ma ka media koho.Ke ʻike ʻia nā papa i ka lā ma o kahi ʻano aniani, he translucent kēia mau kolone a me ka waihoʻoluʻu meli haʻahaʻa.Ke nānā ʻia mai luna mai, ʻike ʻia nā kolone convex a keʻokeʻo momi.ʻO nā kolo ma hope e nui a ʻeleʻele iki.
2.Nucleic acid Keʻano
He mea maʻalahi ka PCR no ka ʻike ʻana i ka brucellosis.Ua hoʻomohala ʻia nā hoʻokolohua PCR he nui no ka ʻike ʻana iā Brucella e hoʻomaikaʻi i ka hiki ke diagnostic.Ua lawa ka ho'āʻo PCR kiko'ī kiko'ī i ka ʻike maʻalahi o Brucella.
3.Serological diagnostics
Nui nā hoʻokolohua serological.ʻO nā hoʻāʻo serological i hoʻohana mau ʻia e hoʻāʻo i kēlā me kēia pipi a i ʻole nā helo pū kekahi me ka buffered Brucella antigen test, complement fixation, indirect or competitive enzyme-linked immunosorbent assays (ELISA) a me ka fluorescence assay.
