Hōʻuluʻulu manaʻo | hoʻohana ʻia e ʻike i ka antigen Avian leukosis P27 i ke koko avian, feces, cloaca, a me ka hua keʻokeʻo. |
Kumukānāwai | Hoʻohana ʻia ʻo Avian Leukosis (AL) P27 antigen Elisa kit e ʻike i ka antigen leukosis Avian P27 i loko o ke koko manu, feces, cloaca, a me ka hua keʻokeʻo.
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Nā Huhu ʻIke | ʻAian Leukosis (AL) P27 antigen |
Laʻana | Serum
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Ka nui | 1 pahu = 192 Hoao |
Paʻa a me ka waiho ʻana | 1) Pono e mālama ʻia nā reagents a pau ma 2 ~ 8 ℃.Mai maloʻo. 2) He 12 mahina ka noho ana.E hoʻohana i nā reagents a pau ma mua o ka lā pau o ka pahu.
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ʻO Avian Leukosis (AL) kahi huaʻōlelo hui no nā maʻi like ʻole e pili ana i ka maʻi maʻi i ka moa i hoʻokumu ʻia e ka Avian Leukosis Virus (ALV) ma ka ʻohana Retroviridae.Hoʻolaha ʻia kēia maʻi ma ka honua holoʻokoʻa a loaʻa ka nui o ka maʻi maʻi.Hiki iā ia ke kumu i ka make a me ka emaciation i nā moa, e hoʻemi i ka hiki ke hana o ka ʻohana, a ʻo ia kekahi o nā maʻi nui e hoʻopilikia nui i ka ulu ʻana o ka ʻoihana moa.He moʻolelo lōʻihi kēia maʻi a ke loaʻa mau nei i nā hihia hou, e like me ka Avian leukemia virus subgroup J (ALV-J), i ʻike ʻia a ʻike ʻia i ka hopena o 1980s ma UK ma ke ʻano he subtype hou o ka maʻi leukemia avian. pōʻino i ka ʻoihana broiler
Hoʻohana ka pahu i ka hana sandwich ELISA, hoʻomaʻemaʻe ʻia ka anti-avian leukocyte P27 monoclonal antibody i hoʻopaʻa mua ʻia ma nā ʻāpana micro-well enzyme. ʻO ka antigen unbound a me nā mea ʻē aʻe, ua hoʻohui ʻia ka enzyme monoclonal antibody e hoʻopaʻa pono i ka paʻakikī antigen-antibody ma ka pā hoʻāʻo.a laila holoi ʻia, hoʻoneʻe ʻia ka conjugate enzyme unbound, hoʻohui ʻia ka hopena substrate TMB i ka microplate, ʻo ka hōʻailona polū e Enzyme catalysis ʻo ia ka hoʻohālikelike pono o ka ʻike antibody i ka laʻana.Hoʻohui i ka hopena hoʻomaha, Ma hope o ka pane ʻana, ua ana ʻia ka waiwai o ka absorbance A i loko o ka pūnāwai hopena e ka lōʻihi hawewe o 450 nm.
Reagent | Volume 96 Hoao/192 Hoao | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Aʻo | 1 pcs |