1.Add reagent i 100ml wai hāpana, ma hope o ka hoʻoheheʻe, incubate ma 36 ° C no 24h
2. Ka wehewehe ʻana i nā hopena:
kala ʻole = ʻinoʻino
Melemele = maikaʻi no ka huina coliforms
Melemele + fluorescence = Escherichia coli maikaʻi.
KA HOIKE ANA
1. E hoʻohui i nā reagents i ka hāpana wai a hui maikaʻi
2. E ninini i loko o ka pā 51-well quantitative detection plate (quantitative well plate) a i ʻole 97-well quantitative detection plate (quantitative well plate)
3. E hoʻohana i ka mīkini hoʻopaʻa helu helu i hoʻopaʻa ʻia e ka papahana
e hoʻopaʻa i ka disc detection quantitative (quantitative well plate) no ka hoʻopaʻa ʻana a hoʻoulu ʻia ma 36°C no 24h.
ʻO ka moʻomeheu coliform/fecal coliform pale wela ma 44.5°C no 24h he melemele a maikaʻi.
4. Ka wehewehe ʻana i nā hopena:
kala ʻole = ʻinoʻino
Helu melemele = huina maikaʻi coliforms
Melemele + mānoanoa ʻōlinolino = Escherichia coli hōʻike maikaʻi i ka helu pākaukau MPN